Oxidation of D-(-)-Ribose with H20 2 and Lipid Hydroperoxides
نویسندگان
چکیده
D-(-)-ribose was subjected to oxidation with equimolar amounts of H20 2/Fe2+ and 13-hydroperoxy-9-c/s-l l-fra/75-octadecadienoic acid/Fe2+. Smaller carbohydrates, carbonyl compounds, hydroxy acids and polyols were produced. Identification of carbonyl compounds was achieved by trapping with pentafluorobenzylhydroxylamine hydrochloride (PFBHA-HC1). All products were identified by GC/MS after derivatization with N-methyl-N-trimethylsilyltrifluoroacetamide (MSTFA). The reactions were repeated with D-[13Ci]-ribose in order to reveal the mechanism of oxidation. A possible reaction pathway is outlined starting by re moval of hydrogens from C-H bonds.
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